In order to do this, we added different levels of pH into different test tubes with the enzyme (sucrose), and substrate (sucrose), and we then inverted the tube. The Effect of pH on Enzyme Activity pH is a measure of the concentration of hydrogen ions in a solution. Method. However, if conditions become too extreme, the enzyme will denature. ...The Effect of Concentration, pH and Temperature on Enzyme Activity Biology For Majors October 4, 2012 Abstract We examined the reaction an enzyme has when its concentration, pH and temperature are altered. Cause if the environment is to acidic or to basic compared to the natural environment of the enzyme, it could denature the enzyme and prevent it from working. IB curriculum content: Topic 2.5 Enzymes Research question: Does pH have any effect on Enzyme Activity? Factors that disrupt protein structure include temperature and pH; factors that affect catalysts in general include reactant or substrate concentration and catalyst or enzyme concentration. Set up a Bunsen burner, heatproof mat, tripod and gauze. Results showed that the pH stability of soil enzymes was highly dependent on the soils being assayed. Each enzyme has an optimum pH at which the velocity is maximum. Temperature: Temperature could affect the activity of the enzyme (catalase). In these optimum conditions, the rate of reaction is at its maximum. Most of the enzymes are proteinaceous in nature and thus, their activity is often affected by varying concentrations of the substrate, pH, concentration of the substrate, and also the presence of other substances at the site of enzyme activity. Most enzymes function efficiently over a narrow pH range. The activity of an enzyme can be measured by monitoring either the rate at which a substrate disappears or the rate at which a product forms. As seen on the graph, reaction rates fell dramatically as pH deviated from 7. The higher the hydrogen ion concentration, the lower the pH. My Conclusion: The reaction rate is fastest near pH 7 and slower at higher and lower pH. Effect of pH: Increase in the hydrogen ion concentration (pH) considerably influences the enzyme activity and a bell-shaped curve is normally obtained (Fig. denaturation) Temperature, pH and substrate concentration will all influence the rate of activity of an enzyme; Temperature A change in pH above or below this range reduces the rate of enzyme … Various factors may affect the activity of enzymes, by either affecting the frequency of enzyme-substrate collisions or by affecting the capacity for the enzyme and substrate to interact (e.g. pH: pH could affect the activity of the enzyme (catalase). The enzyme displayed very little or no activity at a pH higher than 9 or lower than 6. Discussion The purpose of this experiment was to investigate the effects of pH on catalase activity. This is because all enzymes are proteins, and the extreme conditions will affect the bonds in their tertiary structures. In summary, enzymes work best at their optimum temperature and pH. pH at which an enzyme’s activity is greatest. Most of the enzymes of higher organisms show optimum activity around neutral pH … Background Research: I researched other similar experiments and they studied the enzyme catalase which sped of the breakdown of hydrogen peroxide into water and oxygen. 66.5). Aim/purpose: To figure out if the change in pH affects the speed of enzyme activity. My Strategy: I will prepare test tubes with the same amount of enzyme at different pH, and I will measure the height of the bubbles. 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